Iso-Seq/PacBio Transcriptome Sequencing
Isoform Sequencing (Iso-seq) based on long-read sequencing technology can detect full-length cDNA sequences, from the 5’ end of transcripts to the poly-A tail, and provides accurate information about alternatively spliced exons and transcriptional start sites. By capturing the entire transcript sequence, full-length transcriptome sequencing provides valuable insights into gene regulation, transcript diversity, and functional annotation of the genome.
Features and Advantages
Higher Transcripts Detection with Multi-throughput Iso-Seq
Absolute Quantification with UMI
Poly A analysis with polyA Iso-Seq
Experienced pre-sales and after-sales support
Sequencing Standards
Sequel II Sequencing Platform
≥20 Gb sequencing data per sample is recommended
Sampling Requirements
Total RNA: 1.5 µg ≤ m < 3 µg
RIN ≥7.0
- 28S/18S(23S/16S) ≥1.2
Service Details
Typical 40 working days from sample QC acceptance to sequencing data availability
Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data
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