Stereo-seq
Stereo-seq, a spatio-temporal omics technology captures mRNA from tissue section by stereo chips and restores the spatial context by utilizing the spatial barcode (Coordinate ID, CID), thus establishing a solid research foundation for further understanding the relationship between gene expression, morphology of cells and local environment. Stereo-seq is a pioneering tool that achieves Nanoscale Resolution: can theoretically achieve a 100% cell capture rate, obtaining more informative and accurate cell clustering results.
Features and Advantages
Nanometers Single Cell Resolution
• 500 nm resolution, one of the highest in the market
• Deep learning model to for true single cell segmentation and resolution
• Deep learning model to for true single cell segmentation and resolution
Large Centimetre Level Working Area
• Flexible to suit for tissues of any sizes: Tissue Section Area from 1 cm² up to *169 cm²
• Fits unusual large organ tissues including full macaque brain
• Fits unusual large organ tissues including full macaque brain
Powerful database and table-chart-interactive design for convenient multi-omics data analysis
Comprehensive Data Analysis Software
• ImageStudio: Image quality assessment
• SAW: Sequence alignment; Generation of Spatially resolved GEM
• StereoMap: Hi-res image display; Customized
• SAW: Sequence alignment; Generation of Spatially resolved GEM
• StereoMap: Hi-res image display; Customized
Sequencing Standards
Guaranteed ≥80% of bases with quality score of Q30
DNBSEQ™ PE100 sequencing
Sampling Requirements
Fresh frozen samples are suggested embedded in Tissue-Teck OCT, to avoid RNA degradation, we recommend performing tissue embedding within 30 minutes after resecting and wiping extra fluid
The tissue section should not exceed 80% area coverage of the chip
It is recommended the RNA quality (RIN value) of a tissue sample >7
Service Details
Raw data of 1G reads/sample and SAW analysis are available for delivery
The turnaround time for 4 samples including library construction, sequencing and SAW analysis is about 25 working days; slicing, sample QC, dyeing, and permeabilization steps are not inlcuded in the project execution
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