Whole Genome Bisulfite Sequencing
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Features and Advantages
Accurate analysis of methylation patterns of every cytosine
Generate a complete methylome map for any species
The gold standard for DNA methylation analysis
Single-base resolution: Analyze methylation state of every Cytosine accurately
Landmark research: Suitable for all the species with accurate genome mapping
Sequencing Standards
PE100 or PE150
- Guaranteed 80% of bases with quality score of Q30 (not for lowinput samples)
Sampling Requirements
Genomic DNA, m≥1 μg; concentration≥15 ng/μL
No degradation or partially degradation
- Lowinput: m≥ 1ng
Service Details
Typical 35 working days from sample QC acceptance to data delivery
Expedited services are available, contact your local representative for details
- Reports and output data files are delivered in standard file formats: BAM, .xls, .png and FASTQ data
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